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Effect of CeO2 Nanoparticles on Germination and Total Proteins Pattern of Brassica nigra Seeds

[ Vol. 2 , Issue. 2 ]

Author(s):

Rahul Sakla, R. Hemamalini, Kumar Pranaw and Sunil Kumar Khare   Pages 122 - 126 ( 5 )

Abstract:


Background: Although materials with nano-sized particles have always been prevalent in nature, lately the production and use of synthetic nanoparticles (NPs) in the industry are on the rise. This has led to a concomitant increase in their release into the environment. In the assessment of risks posed to human health it has emerged that plants are the potential entry points for NPs in the food chain.

Objective: The toxic effect of cerium oxide NPs (CeO2 NPs) on seed germination in Brassica nigra was studied viz-a-viz the activity of oxidative enzymes and global proteome expression.

Method: Brassica nigra seeds were grown in the presence of different concentrations of CeO2 NPs. The effect on the activity levels of oxidative enzymes - Superoxide Dismutase (SOD), Glutathione reductase (GR) and Catalase (CAT) and the global proteome expression of the seeds exposed to these NPs were studied.

Result: It was found that the levels of activities of all the oxidative enzymes were elevated to some extent, some even up to 2.5 fold. It emerged from proteome analysis that 14 proteins spots were upregulated, and 19 protein spots were down-regulated. Among others, 202 protein spots were completely absent from the gels of the control, while 154 spots were absent from the gels of the NP treated sample.

Conclusion: Oxidative stress is generated in the Brassica nigra seeds and its proteome is also significantly altered in the presence of CeO2 NPs. Thus, it can be concluded that CeO2 NPs have the potential to cause severe damage to living cells.

Keywords:

Cerium oxide nanoparticles, Brassica nigra, superoxide dismutase, glutathione reductase, catalase, 2-D gel electrophoresis.

Affiliation:

Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.

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